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Freeze-thaw cycling is especially destructive to peptides each cycle forms ice crystals that physically shear the molecule, so even one accidental freeze can tank a vial

Desulfovibrio bacteria enhance alpha-synuclein aggregation in a Caenorhabditis elegans model of Parkinsons disease
found the antitumor effect of shikonin against colorectal cancer cells

doi:10.1016/j.smrv.2018.03.004 Dominguez JE, Krystal AD, Habib AS

HSA and FSA cards are a different story, and the answer is it depends on your plan administrator

They can be, especially when medically supervised
