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This study aims to verify the effects of supplementation with selenium, iron, and vitamin E, on the incidence of diarrhea, oxidative metabolism, blood metabolites, hematological parameters, and performance of calves under immunological challenge with Anaplasma marginale in the suckling phase

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Physiology 35, 302327

Afterwards, cells were lysed by three freeze and thaw cycles in liquid nitrogen and a 37 C waterbath, followed by addition of 900 L resuspension buffer (10 mM MgCl 2 , 10 mM ZnCl 2 , 1 M Tris-HCl, pH 8.0) and warming the samples to 28 C