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Emergency signs requiring immediate medical attention include progressive weakness in the leg, foot drop, or loss of bowel/bladder control (cauda equina syndrome) these require urgent evaluation

The protein concentrations were determined using a commercial kit (BioRad Protein DR Assay Reagent Kit, United States)[13-19]
Theprincipal distinction between methyl-, ethyl-, andn-propyl-cobalamin lies in the relative stability of this MLCT state, with the terminal alkyl groups in ethyl and n-propyl acting as electron donors to destabilize the partial carbon anion in the MLCT state
Severe vitamin B 12 deficiency in a mouse model showed dramatic alterations in the level of DNA methylation in the brain, which might lead to neurologic impairments (103)

True adrenal insufficiency will almost always require hydrocortisone replacement therapy (often life-long)